Codon optimization prevents premature polyadenylation of heterologously-expressed cellulases from termite-gut symbionts in Aspergillus oryzae
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چکیده
منابع مشابه
Speciation of termite gut protists: the role of bacterial symbionts.
At least 12 termite gut protists have been named because of their bacterial symbionts. Dozens more species are diagnosed by epi- and endosymbionts and more still have regular bacterial associations referred to in their species description. Molecular systematic studies have begun to identify these bacteria, but the ecological relations with their protist bionts are still unknown. Recent findings...
متن کاملCharacterization of Aspergillus oryzae aspartyl aminopeptidase expressed in Escherichia coli.
To characterize aspartyl aminopeptidase from Aspergillus oryzae, the recombinant enzyme was expressed in Escherichia coli. The enzyme cleaves N-terminal acidic amino acids. About 30% activity was retained in 20% NaCl. Digestion of defatted soybean by the enzyme resulted in an increase in the glutamic acid content, suggesting that the enzyme is potentially responsible for the release of glutamic...
متن کاملEvidence for Cascades of Perturbation and Adaptation in the Metabolic Genes of Higher Termite Gut Symbionts
UNLABELLED Termites and their gut microbes engage in fascinating dietary mutualisms. Less is known about how these complex symbioses have evolved after first emerging in an insect ancestor over 120 million years ago. Here we examined a bacterial gene, formate dehydrogenase (fdhF), that is key to the mutualism in 8 species of "higher" termite (members of the Termitidae, the youngest and most bio...
متن کاملHydrogen production by termite gut protists: characterization of iron hydrogenases of Parabasalian symbionts of the termite Coptotermes formosanus.
Cellulolytic flagellated protists in the guts of termites produce molecular hydrogen (H(2)) that is emitted by the termites; however, little is known about the physiology and biochemistry of H(2) production from cellulose in the gut symbiotic protists due to their formidable unculturability. In order to understand the molecular basis for H(2) production, we here identified two genes encoding pr...
متن کاملTermite gut flagellates and their bacterial symbionts: Phylogenetic analysis and localization in situ
SSU rRNA genes of oxymonad protists from the hindgut of the wood-feeding termite Reticulitermes flavipes were PCR-amplified using a newly designed oxymonad-specific forward primer and a newly designed reverse primer specific for termite gut flagellates. After cloning, the clone library was sorted into four groups by RFLP analysis, and nearly full-length SSU rRNA gene sequences were obtained for...
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ژورنال
عنوان ژورنال: The Journal of General and Applied Microbiology
سال: 2008
ISSN: 1349-8037,0022-1260
DOI: 10.2323/jgam.54.343